Okay, no. This is not how plot "twists" go. A plot twist is "a radical change in the expected direction or outcome of the plot of a novel, film, television series, comic, video game, or other work of narrative"
Meaning the game has to lead you on for a while, then change.
KOTOR and Revan is a plot twist because the game leads you to believe you are not him. Revan is "dead" and when talking about your character's past, you reference a family and life before joining the military. So when they reveal that none of that is true, its a twist.
Likewise in SH2. You are lead to believe Mary died of natural causes. And when its revealed James killed her, thus plot twist.
Samus Aran being a chick isn't a plot twist because the game never leads you to believe she's not. You're never called he, or named as "Randy". No one says Sir or asks you how's the hammer hanging. It may have been unexpected, but neither is a jump scare and those aren't plot twists.
Likewise Aeris dying. Not a twist so much as a plot point because the game doesn't lead you to believe she can be saved or that she's immortal. She dies but seeings how the game never implied she couldn't be killed, its not really a radical change in direction. Because there was no misdirection! A plot twist would have been Aeris faked her death with Sephiroth's help and has been his wife and partner in crime this whole time.
THAT is a twist.
And GLaDOS...I'm not sure its a twist so much as an ironic quirk of fate. Because she's not on your side so much as against the other dude. Its not like had GLaDOS been a giant T-1000 instead of a potato she wouldn't have killed you. It would have been better to use Wheatley as the bad guy twist, because he's set up to be nice and GLaDOS is his enemy.
And NO mention of the Bioshock twist? Would you kindly fix that.