Best one-liners? (Punchlines, coolness attempts etc. - Not quotes!)

[dasUnbekannte]

Just about anything Glados says in Portal.
"I've got your voice on file here, "... helloooo!" THAT'S YOU. THAT'S HOW DUMB YOU SOUND."

 

[Klepa]

For some reason I love the one-liners from Call of Juarez: Bound in Blood^^

a-dee-os ah-me-goh!

I'll only contribute with the most important one-liner-man in history.

"Blow it out your ass."

 

[skywalkerlion]

Anything Cole Train says.

 

[Vlane]

"I hope you saved enough room for my fist, because I'm going to ram it into your stomach and break your goddamn spine."

Not sure if that counts.

 

[Safety 3rd]

GTA:IV

Narrowly missing a large black lady who was in the autumn of her years only to hear her shout "Botheration" in a comely West Indies accent made me put the controller down until i stopped laughing.

*edit*

Sorry, ignore this one, it's not really a one liner at all.

 

[David_G]

"I don't help people... I kill them."
-Jack from Madworld

"Critical, biatch!"
-Roland from Borderlands

Also, the classic:

"Your right hand comes off?"
-Leon from Resident Evil 4

 

[Solidus00]

Devil May Cry

"I should of been the one to fill your darksoul with lighhhhhhhhhhhhhtttttttttttttt!"

 

[Pariah87]

"Stop thinking police-officer and start thinking Playstation - blow shit up!"

I've no idea where I heard that, but I love it.

I'm pretty sure that's from XXX

 

[Elemental]

"Eat boot." - Marcus in Gears 2
that made me laugh hard XD