I'll take a stab at it
Space Invaders (Space Invaders)
Max (Sam and Max)
Agent 47 (Hitman)
Phoenix Wright (Phoenix Wright: Ace Attorney)
Gordon Freeman (Halflife)
Manny Calavera (Grim Fandango)*
Issun (Okami)*
Duck and Dog (Duck Hunt)
Earthworm Jim (Earthworm Jim)
Sly (Sly Cooper)
Prinny (Disgaea)
Haggar (Final Fight)
Ratchet (Ratchet and Clank)
Sir Arthur (Ghosts and Goblins)
Piranha Plant, Koopa, Bloopers, Boo, Big Mushroom(Mario)
DK Barrel (Donkey Kong Country)
Turret (Portal)
Bomberman (Bomberman)
Bub (Bust-a-Move)
Poison Castle Crasher (Castle Crashers)*
Deadpool (Deadpool)
Spiderman (Spiderman)
Kirby (Kirby)
Zubat (Pokémon)
Mr. Mosquito (Mr. Mosquito)
Dhalsim's arm (Street fighter)**
Ivy's Sword (Soul Caliber)
Inspector Gadget's hand (Inspector Gadget)
Link's Hookshot (Legend of Zelda)
Sonic (Sonic)
Arwing (Starfox)
Big Fang (F-Zero)
Megaman (Megaman)
Slime (Dragon Quest)
*=One I had to look up
**=One I looked up for spelling reference
Space Invaders (Space Invaders)
Max (Sam and Max)
Agent 47 (Hitman)
Phoenix Wright (Phoenix Wright: Ace Attorney)
Gordon Freeman (Halflife)
Manny Calavera (Grim Fandango)*
Issun (Okami)*
Duck and Dog (Duck Hunt)
Earthworm Jim (Earthworm Jim)
Sly (Sly Cooper)
Prinny (Disgaea)
Haggar (Final Fight)
Ratchet (Ratchet and Clank)
Sir Arthur (Ghosts and Goblins)
Piranha Plant, Koopa, Bloopers, Boo, Big Mushroom(Mario)
DK Barrel (Donkey Kong Country)
Turret (Portal)
Bomberman (Bomberman)
Bub (Bust-a-Move)
Poison Castle Crasher (Castle Crashers)*
Deadpool (Deadpool)
Spiderman (Spiderman)
Kirby (Kirby)
Zubat (Pokémon)
Mr. Mosquito (Mr. Mosquito)
Dhalsim's arm (Street fighter)**
Ivy's Sword (Soul Caliber)
Inspector Gadget's hand (Inspector Gadget)
Link's Hookshot (Legend of Zelda)
Sonic (Sonic)
Arwing (Starfox)
Big Fang (F-Zero)
Megaman (Megaman)
Slime (Dragon Quest)
*=One I had to look up
**=One I looked up for spelling reference