The thing is, to win this sort of contest, you have to have done more than eliminate lots of enemies, or take down a hard enemy. All video game heroes have done that. For real machismo, you're almost definitely going to need a talking character, because real machismo is more about attitude, muscles, and how much you sweat than it is about most accomplishments.
Let's see now...
I like Kratos as an option, *love* Duke Nukem (assuming you completely forget about the first few games where he would go on at length about how he liked to watch daytime soaps), but I think overall machismo goes to Conan in the recent game of same name. Between the brutality of all your attacks, the counterattack kills, the constant nude women, the ongoing dialogue about the hatred of civilization, that first boss kill where you slam him into his own hammer... yeah. This is raw, sweaty, meaty MAN.
- J