Having another person mindlessly calling out "Jack" is a tad redundant, so I'll rate them in order. Those I actually *want* dead at the top, those I really, really *don't* want dead at the bottom. ME1 peeps included.
Jack - Didn't even want to recruit her, and was all too glad when she died. She's a great character, admittedly, but I can't stand her.
Ashley - Xenophobic *****! Funnily enough, I did actually get an opportunity to make my dreams come true, here.
Jacob - I kind of liked him, but he was just a stock straight guy, and shallowly characterised.
Samara - Same as Jacob. Straight, not really believable for me, and too cold; a crappy replacement for Liara.
Grunt - Crappy replacement of Wrex. No real character, although it is kind of fun having an bloodthirsty walking tank aboard the ship, I'll give him that.
Don't want people dead past this line.
Kaidan - He reminds me a lot of Carth, especially in that he just does not shut up. Ever. Still, I guess I kind of liked him, although not as much as the KoTOR character that he's based on.
Thane - Kind of cool - actually, really cool, but I had to start making some tough choices here.
Miranda - I like her, and her story, even if she's whiny about her perfection, and sees her commander as the perfect person to lay her daddy issues on. I even tried a romance with her, before I fell for Tali (again. On my fourth playthrough. Fourth consecutive Tali playthrough.)
Garrus - Pretty cool guy, and he had some interesting development in ME1, but if I ever hear the word 'calibrations' again, I might just cry.
Mordin - Awesome mad scientist, and he sings!
Wrex - I never really liked Canderous in ToR, but Wrex was always a key part of my ME1 squad, and he does have a couple of interesting conversations with Shepard.
Legion - All round awesome. I loved the idea of having a Geth onboard, and his different perspective - and contextual dialogue - were both brilliant.
Tali/Liara - Simply can't decide here. I think I'd finally decided to let Liara go until I played LotSB, but now, once more I'm fucked if I know what to do, especially considering that things are going to get a tad awkward in ME3.
Anyway, let me know what you guys think of my list, and how you would change it.