-Tali'Zorah nar Raya vas Neema Vas Normandy,from Mass Effect. I like her voice,and she has a funny personality. And she isn't perfect,or trying to be. I'm pretty sure she's going to be on nearly every male's list here.
-Merrill,from Dragon Age II. I've never even PLAYED the game,but from what I've read and been told,she's a laugh riot,cute as a button,and has a deep character.
-Shale from Dragon Age I. I LOVE her snark. ESPECIALLY when Zevran's nearby. Yes,Shale is a woman,take her to the golem registry.
-Agent Texas,AKA Alpha/Epsilon Allison from Red vs Blue. Snarky,badass,hilarious. She's a hoot to watch in a fight,and she's funny in out-of-combat situations.
-Sarah Kerrigan from Starcraft. Pre and post infestation. She's snarky,confident (especially after infestation),and gets the job done. She was dead serious,and completely right when she said that she was the Queen ***** of the Universe.
-The Genetic Lifeform and Disc Operating System,AKA GLaDOS from Portal. Snarky (okay,that seems to be the biggest thing here...),confident,and just awesome all-in-all.
-Elite Commander Ivanna Dostya from Supreme Commander. Not snarky,but she's ruthless,intelligent,and badass,which is a requirement to be Doctor Brackman's military atachee...
I'm sure there's more,but that's all I can think of off the top of my head.