Any of the Warcraft/Starcraft ones are pretty priceless. I've wasted a lot of time clicking on those units.
"Protect and Serve" ~ Castigar, Dark Reign II, one of my favorite units of all time
"I sense a soul in search of answers." ~ Adria The Witch, Diablo // Protoss Probe, Starcraft
"I am he who hath entrusted his soul to the eternal vortex of time. Ye know me, and if ye do not, ye shall be made to know me. It shall be engraved upon your very soul, Lezard Valeth."
The speech goes on, but I can't remember all of it. I've honestly been considering re-playing the game lately. His quotes in VP2: Silmeria were even better, since he was in your party for real rather than just in the after-dungeon.
"You should leave this place and cower in your home, that is the proper
course for one so weak." Was one of the better ones, though they're all pretty much the same arrogant spiel.
Anything from GLaDOS is nice, though there's no need to reproduce them. The same with all the Andrew Ryan quotes, which have been my away messages over the course of the past few weeks.
"Ours was a proud people, and always the strongest. For thousands of years our empire expanded. For so long, we could imagine ourselves alone in the universe. For so long, never did we encounter advanced life. And we traveled faster and farther, spreading in our galaxy. And before long we could see the day when our reachable systems would have been exploited, and then there would be nowhere else to go. And we discovered subspace. It gave us our galaxy and it gave us the universe. And we saw other advanced life. And we subdued it or we crushed it. In months, the elimination of billions of years of evolution, on a similar but slower path. With subspace, our empire would surely know no boundaries." ~ The Ancients, Freespace
Any of the Aekin Bosche monologues from FS2 are also nice.