Somewhat. If gay dude tried to pick up on me, I'd be weirded out but all in all, their only difference from me is their sexual preference - which is a very small part of what makes a person. I don't treat gay people any different from anyone else.
I will say this however, and I don't let it jade me, but to be perfectly honest in my personal experience the lesbians I've met have been very unprofessional in the workplace (bosses dating underlings who end up having to be transferred, giving other ones more hours, lots of drama, etc.) whereas the guy dudes I worked with have been very professional (one guy in particular, I never knew he was gay and after a few months of working with him I found out the person he'd been calling 'hun' on the phone was a dude).