Ezio: "Salute, Claudia."

[ENKC]

"Ramirez..."

Ramirez! Use your riot shield to take down that helicopter! etc.

I'm still playing NBA 2K11 and still sniggering every time they say "sloppy ballhandling" and "spurtability".

 

[-Ulven-]

THEN PAY WITH YOUR BLOOD D:<

Hated thos guards -.-

 

[Baldry]

"I've fought mudcrabs more fearsome than you!"

 

[Davey Woo]

Overlord
Minion: "Naughty, naughty. Those are MINIONS Sheepies!"

Gears of War 2
Carmine: "I heard there's a shit-load of grubs down there sergeant."
Marcus: "More like ten shit-loads..."

Can't think of any more at the moment.

 

[KindOfnElf]

"STOP POKING ME!!" Warcraft 3 peon

gah i had more but totally blanked >.<

"MAKING TRAIIILLLSSS" The Dryads

EDIT: and my hero from my avatar: "I am blind, not deaf" *sigh* Illidan...

 

[niege]

Here's a lockpick. it might be handy if you the master of unlocking take it with you
Resident Evil in a nutshell

 

[Beryl77]

"Good job, Gordon. Throwing that switch and all. I can see your MIT education really pays for itself."
When I heard that for the first time, I couldn't stop laughing.

 

[Rascarin]

"Tired of your name? BUY A TITLE!"

 

[beema]

AC2. Most hurtful line ever.

Maria Auditore: YOu should find some other outlets.

Ezio: I have plenty of outlets.

Maria: I meant besides vaginas.

That even hurt me.

lol
yeah that one stopped me in my tracks and completely broke immersion
it was so very bad

Things that come to mind are the stuff that units say when you click on them in games like StarCraft or Red Alert. That shit gets stuck in your head easily. I used to always have the Tesla Troopers crap in my head "Fully charged" "Rubber shoes in motion"

The Merchant from RE4 "Heh heh heh Thankyou"

Most recently, stuff from TF2 "MEEEEEDIC" "Thankyou her doctor" etc
of course, one could argue that those were intended to be infectious and memorable.

 

[Natasha_LB]

"That was a close one, a second late and you would have fitted nicely in to a sandwich"

 

[Red Pawn]

"Serviceman Burnside! What is Newton's First Law?"
"Sir! An object in motion stays in motion, sir!"
"No credit for partial answers, maggot!"
"Sir! Unless acted on by an outside force, sir!"
"Damn straight!"

 

[jkvoller]

Dimitri, fire the panzershrek

 

[No_Remainders]

I quite like:

"Power, sex. Sex, Power. They both come down to one thing - fucking others" - Geralt (The Witcher)

 

[teh_Canape]

"you and I, kid

we're going places"

 

[Telris]

SPEHHS MAHREENS!!!
http://www.youtube.com/watch?v=WeCfod1XT7E&feature=related
I love the voice acting in Dawn of War:Soulstorm

 

[Les Awesome]

Jump Double Jump - Homer

 

[Woodsey]

"My name is BoLO SanTOESS-EEE."

Really, someone introducing themselves should not be so memorable.

 

[Geo Da Sponge]

"Look, Rhinos! RHINOES! Our enemies hide in METAL BAWKSES! THE COWADS! THE FOOOOLS! We... We should take away their metal bawkses."

"What took you so long to rescue me?" (Princess comes out of room in a gaurd uniform)
"Aren't you a little short to be a gaurd?"

I love this line so much.

Especially when the PC is a dwarf, which makes her reply with: "Is that supposed to be ironic?"