"... we are going to need bigger guns."
(One of the optional responses from Shepard when he first sees the Thorian in ME1)
"Were your parents related? Like, before they were married?"
(Conker: Conker's Bad Fur Day)
From Armed and Dangerous:
Stig: "Stig learn new trick!"
King Forge: "Oh yes? What's that?"
Stig: "Stig grind teeth into calcium dust!"
King Forge: "Yes, well, stop that."
.. and pretty much every other line in that game
"At this point, I'm pretty much the Queen ***** of the Universe. And not all of your little soldiers or space ships will stand in my way again." - Kerrigan (Starcraft... but you knew that)
"I'm British, you muppet" (Psycho: Crysis Warhead)
(Marcus Fenix and crew tear their way out of a giant worm, covered in blood and guts)
"Control, this is Delta. I... oh, I don't even know where to begin."
(Marcus: Gears of War 2)