Best quote from a game?

[Dr. wonderful]

I have many:

Set: I am Set. Why do you tread my shadow?
Vault Dweller: I am the bringer of death. Fall to your knees and beg for mercy... Or give me a sandwich; I'm pretty hungry

Hey Paragon! Shep, what do you say to people:

I sacrificed hundreds of human lives to save the Destiny Ascension! I unleashed the Rachni on the galaxy! So for your sake, hope your escape plan doesn't hinge on me hesitating to shoot a damn hostage!

Shodan...Goshdamn.

"Look at you, Hacker. A pathetic creature of meat and bone, panting and sweating as you run through my corridors. How can you challenge a perfect, immortal machine?"

Liberty Prime:

"Obstruction detected. Composition: titanium alloy supplemented by photonic resonance barrier. Probability of mission hindrance: ZERO PERCENT!"
"Communism is the very definition of faliure!"
"The last domino falls here!"


There are a lot!

 

[Spawny0908]

MacDougal:"Please sir what are we going to do?"
John:"I'm going to hand you over to them, and watch them tear you limb from limb!"
MacDougal:" WHAT!?"
John:"I'm just kidding"
Red Dead Redemption


Oghren: Ah. Yep. Lot of tension around here.
Alistair: You think so, do you?
Oghren: Know what I do to relieve tension?
Alistair: I hesitate to wonder.
Oghren: I polish the ol' weapon.
Alistair: Really.
Oghren: Yep. Give it a good shine. With a dry rag, then with a little grease.
Alistair: That's disgusting.
Oghren: You're telling me you never gave yer blade the old spit-shine?
Alistair: I think that's private.
Oghren: Really? Sodding Chantry and its rules. i like to do it right out in the open.
Alistair: Where people can see you?
Oghren: Yep.
Alistair: Wait, what are you talking about?
Oghren: What are you talking about?
Alistair: (Sigh) Never mind.
Dragon Age: Origins


Sovereign: ?We impose order on the chaos of organic evolution. You exist because we allow it, and you will end because we demand it.?
Mass Effect

 

[cke]

Pretty much all of Portal, some Portal 2.

"So wake up... mr. Freeman, wake up and.... smell the ashes"

"Kirov reporting!" (and many other RA quotes)

"I'm in the middle of some calibrations."

 

[Pelephant]

Elcor Shakespeare in Mass Effect 2. Hilarious.

 

[superdelux]

"......................................................................................."
-Gordon Freeman

 

[Sarah Kerrigan]

"You have awoken on the Van Braun and something has gone terribly, terribly wrong"

The first line of System SHock 2 that tells you things are gonna go batshit insane very fast.

 

[Pingieking]

A lot of awesome quotes from Bioware games.

Tali: "It was totally worth it." Best line in the entire game. Hell, this is the best video game line of that entire year.

Anders talking about Isabella: "She's like a side dish, she comes with every meal."

And the part where Anders comments about Sebastian wearing a belt with Andraste on it, and says something to the effect of "If I was God, I wouldn't want my wife's face on someone's crotch all day."

And the part where Sandal goes crazy:
Sandal: One day the magic will come back - all of it. Everyone will be just like they were. The shadows will part and the skies will open wide.
Bodahn: Huh? What's this?
Sandal: When he rises, everyone will see.
Bodahn: By the ancestors! What has gotten into you, my boy?
Sandal: ..Enchantment?
Bodahn: That's more like it!

A lot of the banter between Isabella and Aveline:
Isabella: Your're not a real captain. Just a captain of a guard. Now I'M a captain.
Aveline: I guess you would know more about having a lot of men under you.

Isabela: How's marriage been treating you, big girl?
Aveline: It's been good. No, great. I'd forgotten what it was like to be-
Isabela: Flipped ass-over-tits and hammered like a bent nail?
Aveline: Love. To be loved.
Isabela: Oh. Right. Of course.
Aveline: Not that I'm complaining about the other thing.

Isabella: Wait, you've gone 4 years without? You must creak like a rusted hinge.

The catfights between those two are usually gold.

EDIT:
I forgot that line of pure gold from Alistair

Alistair: "Well, I suppose I should get back to the old ball and chain."
Teagan: "You know the queen hates it when you call her that."
Alistair" "No she doesn't; just because she killed an arch demon she doesn't scare me."
Teagan: "You keep telling yourself that, your majesty."

Made so much better by the fact that I was the old ball and chain.

 

[Dango]

"A HOODLUM'S GONNA STEAL MY CUUUUUTENEEEEESS!" -Recette

As well as a bunch of other lines from Recettear: An Item Shop's Tale.

Edit: "Because it's just a big, stupid jellyfish" Commander Shepard
I couldn't stop laughing the first time I heard that.

 

[SplicedUp068]

GLaDOS- "Well, this is the part where he kills us."
Wheatley- "Hello, this is the part where I kill you."
-Portal 2

 

[K4ndY]

*inhale*
SPAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACCCCCCCCCCCCCCCCCCEEEEEEEEEEEEEEEE!!!!!!!!!!!!!!!1!!!!!!!!!!!!
-Space Core, Portal 2

Ninja'ed

Also:
"HEY! LISTEN!!"
-Navi, LoZ OoT

 

[OneEyeX]

"LOSS OF BLOOD! MY ONLY WEAKNESS! HOW DID YOU KNOW!?"
-Magicka

 

[silver wolf009]

"Luke,I am your father" .

On the subject of fatherhood:
"I would have been your daddy, but a dog beat me over the fence."

It just comes out of left field and leaves before you even understand it.

 

[Alphakirby]

"WHAT IS A MAN! A MISERABLE LITTLE PILE OF SECRETS!" -Dracula from Castlevania:Symphony of the Night

I like the quote because 9 times out of 10,it's true.

 

[DarkRyter]

"Look into the dark of humanity, and see what more than a shadowed husk of misunderstandings and desire. What more can be done to change what has already been for all of our history? What point in there trying to escape this unending cycle of self-destruction. For in time, our sins will condense into a thick, corrosive goo, building upon oh- OH SHIT SPY IS A PYRO"

~Some guy I killed in tf2.

 

[GodofDisaster]

I'll say it before and I'll say it again this is my favourite quote.

"Life is a serious battle and we have to use the tools we are given. It's important to master the cards in your hand rather than complain about the ones your opponent was given....Let us begin, contests like this are proof that your really living."

Grimsely from Pokemon Black and White.

Also from Pokemon Plantium.

"This is a nice place you've got here, I've kind of gotten to like it so I'm going to pop in everyday for now on, for no reason at all." (Your rival when he pays a visit to your villa.)

Finally and although I don't think this counts as a quote since it's just a sign but still to me I love it.

"Marvelous brige, truly marvelous and also a bridge."

The sign outside Marvelous bridge in Pokemon Black and white.

 

[AWOL]

GLaDOS- "Well, this is the part where he kills us."
Wheatley- "Hello, this is the part where I kill you."
-Portal 2

^ this had me dying my first time through.

For me, the first thing that comes to mind is a lot of the stuff that Cortana says in Halo 3 I believe. The prophetic/metaphorical stuff like:

"I am your shield. I am your sword."

And all that other good stuff she says when she's captured. And as I happen to be replaying Max Payne at the moment, I've noticed that Max has a ton of awesome dialogue too.

 

[Auninteligentname]

Garrus: You ever miss those talks we had in the elevator?
Tali: No.
Garrus: Come on, do you remember we'd all ask about your life on the flotilla? It was an opportunity to share!
Tali: This conversation is over.
Garrus: Tell me again about your immune system!
Tali: I have a shotgun.
Garrus: Maybe we'll talk later.

That right there is ME2 gold.

 

[Anaklusmos]

I see live people. -gunshots- I see dead people.

Whenever I see a world untouched by war, a world of innocence, a world of lush forests and clear rivers... I really just wanna nuke the crap out of it.

You have something on your face. -shot- Got it.

Actually, that is a grenade in my pants.

I don't have to shoot you, I can run you over!

If the guns are churnin', your ass is burnin'. If the guns are blazin', you ass is... erasin'. I don't know, what do you want from me?

We're hit! We're hit! WE'RE ALL GONNA' DIE! WE'RE.. Haha, just kidding, should have seen the looks on your faces.

Oh, suck it up! You act like you got both arms blown off.

I am a lion among the lambs, and they do not fear... they cannot fear...

They are coming for their revenge.
THEN LET THEM COME, FOR FROSTMOURNE HUNGERS

This is the begging, AND THE END!